Sort Questionnaire

Name:
Phone:
Email:

1.	Will there be more than one cell sort? Yes No If Yes, how many?
2.	What types of cells are you bringing? Are these adherent or suspension cells? What fluorescent probes will you be using?
3.	What is the cell concentration of your samples? Samples should be at a minimum concentration of 5 x 10 to the 6 cells/ml. x 10 to the
4.	What is the total number of cells per sample you are bringing? x 10 to the
5.	How many cells are you expecting to have returned? x 10 to the
6.	What size are the cells in the population you want to collect?
7.	What will you use as a negative control?
8.	Is this a dual label experiment? Yes No If so, do you have single color controls for compensation? Yes No
Comments:

Also please be aware of the following:

Please filter your samples for aggregates or debris with a 40-45 micron mesh filter. Keep in mind that filtration of your cells will tend to decrease your cell number, so make sure you begin with adequate cell numbers. 50-µm Nitex Nylon Mesh (Genesee Scientific Cat #57-106) or 40-µm cell strainer cap (BD Falcon Cat #352340)
Provide your own ice if samples need to be kept on ice.
Provide samples in 5 ml Falcon 12 x 75 polystyrene tubes (BD Labware Cat # 352054).
Provide 12 x 75 collection tubes with 1 ml of media in each tube.
Provide media if your cells need to go back into culture.
If your schedule will delay your arrival for more than 15 minutes, please give us a call.

Thank you for your attention to this information. We appreciate your business and look forward to working with you.